Know Everything About Intra Cytoplasmic Sperm Injection(Icsi) Treatment


The word IVF is known to all, but for those who don’t recognize, it stands for In Vitro Fertilization and is generally used for people having difficulties with perceiving a child. Infertility concerns nearly 13 to 14 per cent of the world population, following to close to one in six pair facing challenges in conceiving. The evolution of IVF can be observed back to the birth of the world’s first test-tube child, Louise Brown, in 1978. Although India was not very behind as on 3rd October 1978, Dr Subash Mukhopadyay attended Kanupriya Agarwal in Kolkata.

In this article, Dr Hrishikesh Pai, who is one of the best IVF specialists, explains about Intra Cytoplasmic Sperm Injection(ICSI) treatment. He also holds excellency in IVF, IMSI, Egg donation, Embryoscopy and explains to us regarding the whole approach and practice of ICSI treatment. At present, he works as a Specialist at Bloom IVF centre, which is a chain of 9 IVF centre and is also known as the best IVF centre in India. Dr Hrishikesh Pai gave us the entire low-down on ICSI treatment, from how it goes, what it makes, and to how to go for it. Here’s everything you want to understand.

What is Intra Cytoplasmic Sperm Injection(ICSI) treatment?

According to Dr Hrishikesh Pai, the process of injecting the sperm cell inside the cytoplasm of the embryo is called as ICSI Treatment (Intra Cytoplasmic Sperm Injection). This surgery is a functional form of In Vitro Fertilisation (IVF) that is done for the cure of critical cases of male-factor infertility. ICSI procedure involves the dose of a single sperm straight into a mature egg. This operation is done in similarity with IVF where, after proper stimulation, the recovered eggs from a woman are combined with the partner’s sperm.

Why would someone need ICSI?


ICSI treatment supports to overcome fertility issues, such as:

  • The male partner gives too few sperm to do unnatural insemination (intrauterine insemination [IUI]) or IVF.
  • The sperm may not transport in the usual fashion.
  • The sperm may have difficulty connecting to the egg.
  • A choke in the male reproductive region may keep sperm from going out.
  • Eggs have not bred by regular IVF, despite of the condition of the sperm.
  • In vitro grown eggs are being used.
  • Earlier frozen eggs are being utilized.

Dr Hrishikesh Pai’s Top Tips and steps for ICSI Treatment?


  • As with regular IVF treatment, you will be given fertility medicines to excite your ovaries to produce several mature eggs for fertilization.
  • When your eggs are active for a gathering, they will be recovered through a small outpatient procedure. The obtained eggs are used quickly or frozen and utilized later on.
  • Meantime, the sperms are obtained from the semen unit of the male partner. These sperms are cleaned, and a single most suitable sperm is taken.
  • This sperm is inserted carefully into the cytoplasm of the egg by applying very thin fine needles.
  • The whole procedure of fertilization is taken out under a powerful microscope.
  • This prepared egg is then put in the incubator. Such an egg starts to show symptoms of fertilization after 24 hrs. The egg proceeds to divide and create an embryo.
  • When the egg is three days old holding eight cells, it is available to be carried to the uterus. Or the change may be made on day five and is termed a Blastocyst transfer.
  • If you’re just doing one embryo transfer (called voluntary single embryo transfer, or SET), doing a blastocyst transfer can increase your chances of a strong, healthy, single child.
  • If all works well, an embryo will stick to your uterus wall and proceed to grow to be your baby. After two weeks, you will be capable of taking a pregnancy test.

Can ICSI affect a baby’s development?


If a lady gets pregnant naturally, there is a 1.5% to 3% possibility that the child will have a significant congenital disability. The chance of congenital disabilities connected with ICSI is related to IVF but somewhat higher than in general conception. The lightly higher risk of congenital disabilities may be because of infertility and not the therapies used to succeed infertility. Several conditions have been linked with the use of ICSI, such as

  • Beckwith-Wiedemann syndrome
  • Angelman syndrome
  • hypospadias
  • sex chromosome anomalies.

They are thought to happen in far less than 1% of babies conceived using this method. Some of the difficulties that cause unproductiveness may be genetic. For instance, male children perceived with the use of ICSI may have related infertility problems as their fathers.


ICSI-IVF appears with all the risks of a natural IVF cycle, but the ICSI method does include additional ones.

A healthy pregnancy comes with a 1.5% to 3% danger of significant congenital disabilities. ICSI surgery displays a slightly higher risk of birth defects, but it’s yet rare.

There is also a lightly increased uncertainty of a male baby having fertility issues in the future. This is because male impotence may be passed on genetically.